GSM3563750: MCF7 WT Vehicle; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7 cell line
esr1 genotype
Wild-type
treatments
Vehicle
chip antibody
ERα (Santa Cruz Biotechnologies, sc543)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and ER-bound DNA complexes were isolated with antibody (Santa Cruz, sc-543). ChIP was performed according to our in-house protocols. A minimum of 10ng DNA was sent to McGill University sequencing core. The library was prepared using TruSeq ChIP Library Preperation kit (Illumina) and was sequenced on Hiseq 2500 (Illumina). Over 16M single end 50bp reads were allocated for each sample.